An honest scientific definition of life is harder than it sounds. The textbook list — metabolism, reproduction, response to stimuli, growth, homeostasis — describes Earthly biology, not life-in-general; we have a sample size of one. Habitability and biosignatures — the questions the exoplanet field now organizes itself around — therefore take a deliberate compromise. They use Earth as the only working example, liquid surface water as the proxy for habitability, and chemical disequilibrium in atmospheric spectra as the proxy for biological activity. The compromise is provisional, the targets are concrete, and as of 2024 the search has identified zero confirmed biosignatures and one contested candidate.
The habitable zone is the orbital band around a star where a rocky planet's radiative balance — incoming starlight, outgoing thermal radiation, atmospheric greenhouse — permits liquid water on its surface. The conservative HZ assumes Earth-like atmospheric chemistry; the optimistic HZ admits CO₂-dominated atmospheres on the cold side and water-vapor-dominated on the warm side. For the Sun: ~0.95–1.4 AU conservatively, ~0.75–1.8 AU optimistically. The complication is the M-dwarf habitability question. M-dwarfs are the most numerous stars (~75% of the Milky Way) and host most catalogued habitable-zone planets — including TRAPPIST-1's seven worlds and Proxima Centauri b. But planets so close to an M-dwarf are typically tidally locked, exposed to flares that can strip atmospheres, and bombarded by UV during the star's long pre-main-sequence phase. The non-detection of atmospheres on TRAPPIST-1b and -1c by JWST in 2023 has tightened the question.
A biosignature is a chemical feature hard to explain without a biological source. The strongest pattern is chemical disequilibrium — gases that should react and disappear over geological time but persist. Earth's atmosphere is biosignature-rich because of the coexistence of O₂ and CH₄: these gases react readily, and their persistent simultaneous presence at percent and ppm levels would be very difficult to explain without photosynthetic and methanogenic biospheres. Other candidates — ozone, N₂O, methyl chloride, the controversial dimethyl sulfide (DMS) — each carry abiotic false positive concerns. The 2023 K2-18b DMS claim sits at the edge of JWST's sensitivity, has been challenged on noise grounds, and is being re-observed. As of 2024, no biosignature has been confidently identified.
The Habitable Worlds Observatory (NASA, late 2030s) is the planned flagship successor to JWST, designed for direct imaging and atmospheric characterization of Earth-like planets in habitable zones of nearby sun-like stars; the goal is roughly twenty-five such atmospheres characterized by the mid-2040s, with biosignature detection as a primary objective. The Drake equation (Frank Drake, 1961) — a multiplication of factors from star-formation rate to civilization lifetime — is still the framework used to put a number on the question, with factors that run from increasingly well-constrained (planet fraction is near unity) to wildly uncertain. The Fermi paradox frames present ignorance: if life is common, where is everybody? The honest scientific position remains deep uncertainty. What has changed is that the question now has instruments aimed at it.